Protein_Domain

Part:BBa_K1848004:Design

Designed by: Samuel Magaziner   Group: iGEM15_Columbia_NYC   (2015-09-18)


Human Gut Hormone Glp1 (7-37) with upstream signal peptide PhoA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Notably, concentration of secreted protein is much lower than internal concentration. As such supernatant need be concentrated before protein screening occurs.


Source

PhoA comes from E. coli strain K12.

References